Paul Reubens hosts the entire show in character as Pee-wee Herman, appearing as Pee-wee in 7 sketches (including the monologue) and performs a song during the good nights, all the while wearing the same outfit.
One of Lorne Michaels' future Kids in the Hall - Bruce McCulloch - appears in the Just Say No to the Army skit.
Sen. Bob Kerrey (D-Neb.) appears in the studio audience during the Diana Ross portion of the Pee Wee Herman's Thanksgiving Special sketch next to actress Debra Winger and former cast member Robin Duke.