If the player encounters the Green Toad in any galaxy where he's digging with a pickax, zooms in his face and shoots him with a Star Bit, his facial expression changes from happy to sad. He will remain sad until the galaxy is complete, or if the player loses a life. He is the only Toad who does this.
First appearance of Rosalina. She has since been a recurring character in Mario games.
The Star Bits that the Lumas love to eat are based off of a type of Japanese candy called "konpeitô".
Rosalina is known as "Rosetta" in the Japanese version, and the Lumas are called "Chiko".
The first time Luigi appears in a mainline 3D Mario video game, and as a playable character.