Chizuru Yukimura is in Kyota looking for her father, a doctor who's gone missing. When demons called ronin chase her, she is saved by the Shinsengumi. They allow her to live at their headquarters since they are also looking for her father.
Chizuru becomes more familiar with the inner workings of the Shinsengumi society. Following a lead concerning the whereabouts of her father, she ends up running into the Shinsengumi's enemy. This encounter leads to uncovering a plot against Kyoto and the Emperor.
It is unclear if Sanaan will recover from his horrible reaction to the medicine. While on patrol, Chizuru and the soldiers rescue a girl who looks exactly like Chizuru. The Oni strangers attempt to kidnap Chizuru.
After the incident of an uncovered Shinsengumi, Kashitarou Itou announces his leave. However, he's not going alone. Meanwhile, the devils fabricate a plan to kidnap Chizuru.