Just after Eric drops Nicole off with Isaac, the clock shows 12:35 and then 12:40, but about 5 minutes later as the scene starts with Nicole talking to Eric, the clock shows 12:30.
New CTU analyst Mariana Stiles says her cousin was Edgar Stiles. Fans of the original show would know that Edgar Stiles was a reoccurring analyst who was friends with Chloe O'Brian.
The room the school students are watching the video about the creation of the atomic bomb is room 204... this could be said as "20 4", or "Twenty Four"... "24"