First animated appearance of the Nebolon-B (medical) frigate from Star Wars: Episode V - The Empire Strikes Back (1980), the Mon Calamari cruiser from Star Wars: Episode VI - Return of the Jedi (1983) and the first time the TIE Defender is seen in action.
The space debris in which the Ghost hides during the opening scenes features the logo the Separatists used during the Clone Wars on one large piece. Several of the larger pieces also clearly resemble parts of a Separatist ship, either a dreadnought or a frigate.
When Mon Mothma's speech is being broadcast on the view-screen in Old Jho's Pit Stop on Lothal, the Aurebesh letters on top right of the screen translate to 'Live Feed'.
The Ghorman Massacre mentioned by Mon Mothma during her first, holographic speech was first mentioned in the 1990 Rebel Alliance Sourcebook published by West End Games.
Genevieve O'Reilly reprises her role as Senator Mon Mothma from Episode III - Revenge of the Sith (2005) and Rogue One (2016).